Three-dimensional (3D) bioprinting is transforming skin regeneration and cancer research by allowing complex biological structures to be accurately fabricated layer by layer. This strategy encourages the optimum tissue architecture for oncology and regenerative medicine by allowing for the customization of cell types, extracellular matrix components, and vascular networks. Wound dressings are crucial for wound management, and nanotechnology is revolutionizing the field by promoting wound healing by combating bacterial infections. Nanomaterials like nanoparticles, hydrogels, nanofibers, and nanocomposites can accelerate healing by promoting cell migration, proliferation, differentiation, and modulating the inflammatory response. Furthermore, nanoscale stem cell therapy breakthroughs herald the crisis moment in regenerative medicine, efficiently resolving challenges and opening up new options for clinical applications.